Unexplained infertility tests in Pune 2026 include sperm DNA fragmentation analysis, ERA (Endometrial Receptivity Array), diagnostic hysteroscopy, PGT-A embryo testing and EMMA/ALICE endometrial microbiome testing. These advanced diagnostics, available at IRSA Fertility in Kharadi, Pune, identify the hidden causes missed by standard semen analysis, HSG and hormone panels — giving couples a specific diagnosis and a targeted treatment plan.

KEY TAKEAWAYS

Unexplained infertility — no identifiable cause after standard testing — affects 25 to 30 percent of infertile couples in India.

Standard fertility tests (semen analysis, HSG, hormones) miss a significant proportion of causes that advanced diagnostics can detect.

Sperm DNA fragmentation, ERA testing, hysteroscopy and PGT-A are the four highest-yield advanced tests for unexplained infertility in Pune.

EMMA and ALICE endometrial microbiome tests can identify uterine environment disruption that standard tests cannot detect.

IVF itself is diagnostic — it shows how eggs fertilise, how embryos develop and whether implantation occurs — information unavailable from any other test.

Dr. Shitole’s clinic at Kharadi, Pune offers a structured advanced infertility investigation panel that systematically addresses unexplained infertility.

Unexplained Infertility in Pune: What Tests Should You Get in 2026?

Unexplained infertility tests in Pune 2026 are in high demand among the growing proportion of couples who have completed standard fertility investigations — semen analysis, HSG, ovarian reserve testing, pelvic ultrasound, basic blood tests — and been told everything is ‘normal’, yet cannot conceive. This diagnosis affects 25 to 30 percent of infertile couples in India (ICMR 2024).

The frustration of unexplained infertility is real and legitimate. But ‘unexplained’ does not mean untreatable or unfindable — it means the standard investigation panel has not yet identified the cause. Advanced diagnostics, available at Dr. Rajendra Shitole’s clinic at IRSA Fertility, Kharadi, Pune, can detect subtle factors that standard tests miss entirely. This guide explains exactly which tests to get, what they look for and what they cost in Pune.

Quick Facts

Fact	Data
Unexplained Infertility Prevalence in India	25 to 30 percent of infertile couples
Sperm DNA Fragmentation Test Cost Pune	INR 3,000 to 6,000
ERA Test Cost Pune	INR 25,000 to 40,000
PGT-A Cost Pune	INR 30,000 to 50,000
Hysteroscopy Cost Pune	INR 8,000 to 20,000
EMMA and ALICE Tests Cost	INR 25,000 to 45,000

Pune Unexplained Infertility Statistics 2025-2026

Metric	Data Point	Source
Unexplained infertility prevalence	25 to 30 percent of infertile couples	ICMR 2024
Couples with abnormal ERA results	Approx. 25 percent	PubMed 2022
Sperm DNA fragmentation in unexplained infertility	Up to 25 percent of men	PubMed 2021
Uterine abnormality found on hysteroscopy in UI	15 to 20 percent of cases	Industry estimate
Endometrial microbiome imbalance in implantation failure	15 to 25 percent of cases	Industry estimate
IVF success rate in unexplained infertility (under 35)	55 to 65 percent per cycle	Industry estimate
Chromosomal embryo abnormality in unexplained infertility	Higher than standard population	Industry estimate

The Standard Tests — What Has Already Been Done

Before considering advanced diagnostics, it helps to confirm that the standard infertility panel is complete. For women: AMH, Day 2 FSH and LH, TSH, prolactin, pelvic ultrasound (ovarian reserve, uterine structure), HSG (tubal patency). For men: full semen analysis (count, motility, morphology, volume). If any of these are incomplete, start there.

If all standard tests are normal and 12 months (or 6 months if over 35) have passed without conception, it is time to move to advanced investigation at a specialist clinic like IRSA Fertility in Kharadi, Pune.

Advanced Test 1 -Sperm DNA Fragmentation (DFI)

Sperm DNA fragmentation is the most commonly overlooked cause of unexplained infertility. A standard semen analysis looks normal — but the sperm’s DNA is damaged, preventing proper embryo development. High DFI (above 25 to 30 percent) is associated with fertilisation failure, poor embryo quality, early miscarriage and IVF failure even with good morphology.

The DFI test costs INR 3,000 to 6,000 at Dr. Shitole’s Kharadi clinic. Results are available within 24 to 48 hours. Treatment options for high DFI include antioxidant supplements for 3 months, TESA sperm (testicular sperm has lower fragmentation) and modified IVF protocols.

Advanced Test 2 -ERA (Endometrial Receptivity Array)

The ERA test analyses gene expression in a small endometrial biopsy to identify the personalised Window of Implantation (WOI). In approximately 25 percent of women, the WOI is displaced from the standard Day 5 post-ovulation timing (PubMed 2022). Transferring an embryo at the wrong time — even a perfect blastocyst — results in implantation failure.

ERA cost in Pune: INR 25,000 to 40,000. It is done in a mock FET cycle — a biopsy is taken and analysed before any real embryo transfer. Subsequent transfers are timed to the personalised window. ERA-guided transfers have been shown to significantly improve implantation rates in women with recurrent implantation failure.

Advanced Test 3 – Hysteroscopy

Even in women with a normal pelvic ultrasound, hysteroscopy can find subtle uterine abnormalities: small polyps (under 1 cm, invisible on scan), mild adhesions, a partial uterine septum or abnormal endometrial vascularity. These findings, if corrected, can transform IVF outcomes.

Hysteroscopy cost in Pune: INR 8,000 to 20,000. It is a 20-minute day procedure. Dr. Shitole recommends hysteroscopy for all women with unexplained infertility who have not had one recently, particularly before a first IVF attempt.

Advanced Test 4 – PGT-A (Preimplantation Genetic Testing for Aneuploidies)

PGT-A screens embryos for chromosomal abnormalities before transfer. In unexplained infertility, a higher-than-expected proportion of embryos may be chromosomally abnormal — appearing perfect under the microscope but carrying errors that prevent implantation or cause early miscarriage.

PGT-A is done during IVF — a few cells are biopsied from each blastocyst and sent for chromosomal analysis. Only euploid (normal) embryos are transferred. PGT-A cost in Pune: INR 30,000 to 50,000 per batch of embryos tested.

Advanced Test 5 – EMMA and ALICE (Endometrial Microbiome Tests)

EMMA (Endometrial Microbiome Metagenomic Analysis) and ALICE (Analysis of Infectious Chronic Endometritis) analyse the bacterial environment of the uterus. An imbalanced endometrial microbiome — either too little of the beneficial Lactobacillus species or the presence of pathogenic bacteria — reduces implantation rates significantly.

These tests are done on the same endometrial biopsy as ERA. If imbalance is found, targeted antibiotic or probiotic treatment is given before the next embryo transfer. EMMA and ALICE are available at Dr. Shitole’s IRSA Fertility clinic in Kharadi, Pune for INR 25,000 to 45,000.

Advanced Test 6 -Karyotyping Both Partners

In unexplained infertility — particularly with recurrent failure or miscarriage — chromosomal structural abnormalities in either partner can be the underlying cause. Balanced translocations or inversions are ‘balanced’ in the carrier but produce unbalanced embryos that fail to implant or miscarry. Karyotype cost in Pune: INR 2,000 to 5,000 per person.

Advanced Infertility Tests — Summary and Cost Guide

Test	What It Detects	When to Consider	Cost in Pune (INR)
Sperm DNA fragmentation	Hidden DNA damage in sperm	First advanced test for all UI couples	3,000 to 6,000
ERA test	Displaced implantation window	After failed embryo transfer(s)	25,000 to 40,000
Hysteroscopy	Subtle uterine abnormalities	Before first IVF in UI	8,000 to 20,000
PGT-A	Chromosomally abnormal embryos	During IVF for UI couples	30,000 to 50,000
EMMA and ALICE	Endometrial microbiome imbalance	After 2 or more failed transfers	25,000 to 45,000
Karyotyping	Chromosomal structural defects	Recurrent failure or miscarriage	2,000 to 5,000 per person

IVF as a Diagnostic and Therapeutic Tool for Unexplained Infertility in Pune

IVF is not just treatment for unexplained infertility — it’s the most comprehensive diagnostic test available. During an IVF cycle, Dr. Shitole’s embryology team at IRSA Fertility observes egg maturity rates, fertilisation rates, embryo cleavage quality and blastocyst development. Each of these data points narrows the diagnostic picture in ways that no blood test or scan can replicate.

A couple who conceive on their first IVF attempt confirm that the issue was one of sperm-egg meeting (tubal or timing). A couple whose embryos consistently arrest at Day 3 know the problem is embryo development quality. Each cycle generates information that improves the next.

Unexplained Infertility Investigation in Kharadi and Pune

Pune’s working professional community — spanning Kharadi IT Park, Viman Nagar, Kalyani Nagar, Hadapsar, Wagholi, Koregaon Park and the western corridor including Hinjewadi, Wakad and Baner — tends to defer fertility investigation until well into their 30s, often after 2 to 3 years of trying. By that point, the urgency to find answers is high and patience for a repeat standard workup is low.

Dr. Shitole’s is best ivf specilaist in pune with advanced investigation panel at IRSA Fertility, Kharadi can be completed within one menstrual cycle and provides actionable data within 2 to 4 weeks. Couples from Viman Nagar, Nagar Road, Wagholi, Hadapsar and Magarpatta — as well as those travelling from PCMC, Hinjewadi and Baner — come specifically because of Dr. Rajendra Shitole’s reputation for thorough investigation and personalised protocol design. Kharadi’s central position on the Pune ring road makes the clinic accessible from both the east and west corridors of the city.

Frequently Asked Questions

Q: What does unexplained infertility mean?

A: Unexplained infertility means that standard fertility tests — semen analysis, HSG, ovarian reserve, hormones, ultrasound — are all within normal limits but pregnancy has not occurred after 12 months of trying (6 months if over 35). It affects 25 to 30 percent of infertile couples (ICMR 2024) and requires advanced investigation to identify subtle causes.

Q: What are the best unexplained infertility tests in Pune?

A: At IRSA Fertility, Kharadi, Pune, the highest-yield tests are: sperm DNA fragmentation for men, and hysteroscopy plus ERA testing for women. Together these four tests identify a specific cause in the majority of cases. PGT-A and EMMA/ALICE are added for couples with a history of failed transfers.

Q: What is the ERA test cost in Pune?

A: ERA test cost in Pune at IRSA Fertility is INR 25,000 to 40,000. It identifies your personalised window of implantation — about 25 percent of women have a displaced WOI that requires a modified transfer schedule (PubMed 2022).

Q: Should I go straight to IVF with unexplained infertility?

A: For couples under 35, 2 to 3 IUI cycles are a reasonable first step before IVF. For couples over 35 or with a longer infertility duration, proceeding directly to IVF with advanced diagnostics (PGT-A, ERA) is often more efficient. Dr. Shitole will advise based on your age, infertility duration and previous treatment history.

Q: What is the EMMA and ALICE test?

A: EMMA analyses the bacterial composition of the uterine lining to check for microbiome balance. ALICE checks for chronic endometritis (an often symptomless uterine infection). Both are done on the same endometrial biopsy. Abnormal results are treated with targeted antibiotics or probiotics before the next transfer. Available at IRSA Fertility, Kharadi, Pune.

Q: Can unexplained infertility resolve on its own?

A: For younger couples (under 30) with a short infertility duration (under 12 months), spontaneous conception is possible with expectant management. For couples over 35 or with longer duration, expectant management is not recommended — time is a meaningful factor and treatment is more efficient than waiting.